Journal: International Journal of Molecular Sciences

Article Title: FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice

doi: 10.3390/ijms232314979

Figure Lengend Snippet: FOXG1 has no ability to induce the production of oligodendrocytes in adult hippocampal DG of the Foxg1 genotype mice. ( A ) Expression patterns of oligodendrocytes in the hippocampal DG of Foxg1 genotype mice were assessed with immunofluorescence. Red = Oligo2; Green = EGFP-labeled FOXG1; Blue = DAPI. Scale bars = 100 μm. ( B ) Levels of total oligodendrocytes in adult hippocampus are showed as %Oligo2 + /DAPI + cells. ( C ) Levels of endogenous oligodendrocytes in adult hippocampus are showed as %GFP − Oligo2 + /DAPI + cells. ( D ) Expression patterns and ( E ) qualification of total oligodendrocytes in adult hippocampus were studied with immunohischemisitry. Scale bars = 100 μm. ( F , G ) Hippocampal lysates of the two genotype mice were immunoblotted using an antibody against Oligo2. Tubulin was used as the loading control. Values are expressed as means ± S. E. M. n = 6/group. ns—not significant.

Article Snippet: To visualize protein expression, sections were first incubated with primary antibodies, including rabbit anti-GFAP (1:200, Wanleibio, cat # WL0836, Shenyang, China), mouse anti-GFAP (1:200, Proteintech, cat# 60190-1-lg, Rosemont, USA), EGFP (1:200, Beyotime, cat # AG281, Shanghai, China), brain lipid binding protein (BLBP) (1:200, Proteintech, cat # 51010-1-AP, Rosemont, USA), Proliferating Cell Nuclear Antigen (PCNA) (1:250, Proteintech, cat # 10205-2-AP, Rosemont, USA), Tbr2 (1:200, Bioss, cat # bs-11331R, Beijing, China), Oligo2 (1:200, Bioss, cat # bs-11194R, Beijing, China), NeuN (1:1200, Bioss, cat # bs-10394R, Beijing, China) and Dcx (1:200, Proteintech, cat # 13925-1-AP, Rosemont, USA).

Techniques: Expressing, Immunofluorescence, Labeling

Journal: International Journal of Molecular Sciences

Article Title: High Dose Pharmaceutical Grade Biotin (MD1003) Accelerates Differentiation of Murine and Grafted Human Oligodendrocyte Progenitor Cells In Vivo

doi: 10.3390/ijms232415733

Figure Lengend Snippet: List of primary antibodies used for in vivo cell characterization.

Article Snippet: Rabbit anti-Olig2 , 1:400 , Millipore (AB910).

Techniques: In Vivo

Journal: Cells

Article Title: Contribution of Oligodendrocytes, Microglia, and Astrocytes to Myelin Debris Uptake in an Explant Model of Inflammatory Demyelination in Rats

doi: 10.3390/cells12172203

Figure Lengend Snippet: Coexpression of Olig2 with LRP1 and MBP in the CA1 region during LPS + IFN-γ-induced demyelination. ( A ) Confocal images displaying coexpression of Olig2 (green) and LRP1 (red) in explants under control conditions and after LPS + IFN-γ treatment for 1 day. Nuclei are stained with DAPI (blue). Arrows point to intensely stained Olig2 + /LRP1 + cells at 1 day. Scale bars: 20 μm. B, left panels, Representative confocal images showing Olig2 (green) and MBP (red) coexpression under control conditions and after LPS + IFN-γ exposure for 1, 2, and 3 days. ( B ) right panels, Higher magnification images displaying representative double-labeled Olig2 + /MBP + cells observed at day 1, day 2, and day 3. Note the accumulation of MBP immunoreactivity at peri-membrane soma sites of Olig2 + cells at day 2 and within the cytosol at day 3. Scale bars: 20 μm. ( C ) Quantitative analysis of double-labeled LRP1 + /Olig2 + cells in slices under control conditions and after LPS + IFN-γ treatment for 1 day. The values represent the mean ± S.E.M. (n = 3). * p < 0.05, LPS + IFN-γ versus control. ( D ) Quantitative analysis of the number of double-labeled Olig2 + /MBP + scored according to somatic MBP immunoreactivity at day 1, day 2, and day 3. The values represent the mean ± S.E.M. (n = 3). * p < 0.05, peri-MBP + versus peri-MBP ++ and cyto-MBP at 1 day; * p < 0.05, peri-MBP ++ versus peri-MBP + and cyto-MBP at 2 days; * p < 0.05, cyto-MBP versus peri-MBP + and peri-MBP ++ at 3 days.

Article Snippet: After fixation and blocking, slices were incubated with the indicated primary antibodies for 48 h at 4 °C: rat monoclonal anti-MBP (1:1000, Merck-Life Science S.r.l., Milan, Italy), rabbit polyclonal anti-Olig2 (1:200, Merck-Life Science Merck-Life Science S.r.l., Milan, Italy), rabbit polyclonal anti-NG2 (1:400, Merck-Life Science S.r.l., Milan, Italy), rabbit polyclonal anti-Iba1 (1:2000, FUJIFILM Wako Chemicals, Osaka, Japan), rabbit polyclonal anti-GFAP (1:1000, Merck-Life Science, Merck-Life Science S.r.l., Milan, Italy), and mouse monoclonal anti-low density lipoprotein receptor-related protein 1 (LRP1, 1:200 Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Staining, Labeling, Membrane